EMERGING TECHNOLOGY AND ITS IMPACT ON THE PRODUCTION AND QUALITY OF LOCAL TELEVISION NEWS

Technology is an integral part of journalism. Journalists use computers, IP-based servers, and digital gear as instruments to gather, process, and distribute news regarding events that impact our lives. Beyond the basic tools of the trade, the use of technology can dramatically influence the process of producing news. This project examines how the use of this technology impacts the collection, analysis, production, and quality of local television news. The study also addresses how technology is redefining the role of newsworkers in local television newsrooms. The methods of data collection include a survey and focus group of journalists with experience at local and national news networks, a content analysis of sixty local newscasts, and interviews with television news workers. The research questions address the impact of technology on the production and quality of local television news. I ask, among other questions, whether the increased number of required daily tasks has affected quality and whether news professionals say they see such effects; how, and to what extent, digital technologies such as IP-based store-and-forward technology, smaller portable newsgathering gear, and cellular-based transmission methods have affected production, and whether journalists say they see negative effects; that the use of network-affiliate and subscription-based news services have changed how local television news is produced. Overall, I conclude that the role of a local television journalist has changed and a new hybrid editorial/technical role has emerged. These hybrid journalists are required to perform more technical tasks, resulting in time diverted away from traditional tasks such as story research, news analysis, and script writing. My research aligns with existing literature in proposing that these changes are contributing to a decline of quality in local television news. This is an indirect result of using newer technology. The use of these tools has made the collection and distribution of content more efficient, but the speed and ease of this technology have resulted in more tasks being performed by individual journalists, which leads to less locally generated content. In addition, the increased need to supply original content to station websites and social media platforms has also negatively affected quality

The structure of the bacterial oxidoreductase enzyme DsbA in complex with a peptide reveals a basis for substrate specificity in the catalytic cycle of DsbA enzymes

Oxidative protein folding in Gram-negative bacteria results in the formation of disulfide bonds between pairs of cysteine residues. This is a multistep process in which the dithiol-disulfide oxidoreductase enzyme, DsbA, plays a central role. The structure of DsbA comprises an all helical domain of unknown function and a thioredoxin domain, where active site cysteines shuttle between an oxidized, substrate-bound, reduced form and a DsbB-bound form, where DsbB is a membrane protein that reoxidizes DsbA. Most DsbA enzymes interact with a wide variety of reduced substrates and show little specificity. However, a number of DsbA enzymes have now been identified that have narrow substrate repertoires and appear to interact specifically with a smaller number of substrates. The transient nature of the DsbA-substrate complex has hampered our understanding of the factors that govern the interaction of DsbA enzymes with their substrates. Here we report the crystal structure of a complex between Escherichia coli DsbA and a peptide with a sequence derived from a substrate. The binding site identified in the DsbA-peptide complex was distinct from that observed for DsbB in the DsbA-DsbB complex. The structure revealed details of the DsbA-peptide interaction and suggested a mechanism by which DsbA can simultaneously show broad specificity for substrates yet exhibit specificity for DsbB. This mode of binding was supported by solution nuclear magnetic resonance data as well as functional data, which demonstrated that the substrate specificity of DsbA could be modified via changes at the binding interface identified in the structure of the comple

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements